Journal: bioRxiv
Article Title: A parabrachial hub for the prioritization of survival behavior
doi: 10.1101/2024.02.26.582069
Figure Lengend Snippet: ( A ) Formalin was injected into the dorsal surface of the hindpaw, resulting in an initial acute phase (Phase 1, 0-5 min) and a sustained phase (Phase 2, 15-60 min). ( B ) Time spent licking paw in 5 min bins after an injection of formalin (n=9) showing initial and sustained phases. ( C ) Complete Freund’s Adjuvant (CFA) was used as a model of persistent inflammatory pain and spared nerve injury (SNI) was used as a model of neuropathic pain. The von Frey test was used to assess mechanical allodynia in both models and the acetone test was used to test cold allodynia after SNI. ( D ) Timeline for 24 h food deprivation experiments. ( E ) Time licking paw during Phase 2 in ad libitum fed mice (grey) and food deprived mice (blue, n=7-9/group, unpaired t-test, p<0.01). ( F ) Withdrawal threshold to mechanical stimuli before CFA (baseline, grey), ad libitum fed mice after CFA (light blue), and 24 h food deprived mice after CFA (dark blue, n=15, repeated measures one-way ANOVA, p<0.001). ( G ) Withdrawal duration after acetone application before SNI (baseline, grey), ad libitum fed mice after SNI (light blue), and 24 h food deprived mice after SNI (dark blue, n=8, repeated measures one-way ANOVA, p<0.001). ( H ) Timeline for polyethylene glycol (PEG)-induced thirst (30%, 20ul/g body weight s.c.) experiments. ( I ) Time licking paw during Phase 2 in saline treated mice (grey) and PEG treated mice (blue, n=9/group, unpaired t-test, p<0.01). ( J ) Withdrawal threshold to mechanical stimuli before CFA (baseline, grey), control mice treated with saline after CFA (light blue), and mice treated with PEG after CFA (dark blue, n=8, repeated measures one-way ANOVA, p<0.01). ( K ) Withdrawal duration after acetone application before SNI (baseline, grey), control mice treated with saline after SNI (light blue), and mice treated with PEG after SNI (dark blue, n=10, repeated measures one-way ANOVA, p<0.001). ( L ) Timeline for experiments during innate fear (exposure to Trimethylthiazoline (TMT, fox odor)). ( M ) Time licking paw during Phase 2 in mice with control PBS in the chamber (grey) and TMT in the chamber (blue, n=8/group, unpaired t-test, p<0.01). ( N ) Timeline of experiments during conditioned fear. Conditioning involved pairing a shock with a context on day 1. On day 2, mice were injected with formalin and then placed in either the conditioned or novel context 20 minutes after the injection. Paw licking was scored for 10 min during the inflammatory phase. ( O ) Time licking 20-30 min after formalin injection while animals were in either in the unshocked context (grey) or the shocked context (blue, n=10-12/group, unpaired t-test, p<0.05). Data are expressed as mean ± SEM. Grey dots represent individual mice in between subject experiments and grey lines represent individual mice in within subjects experiments. T-test and post-hoc comparisons: *p<0.05, **p<0.01, ***p<0.001. ANOVA main effect of group: ☼☼p<0.01, ☼☼☼p<0.001
Article Snippet: Mice were placed in a fear conditioning chamber (HABITEST modular behavioral test system, Coulbourn Instruments).
Techniques: Injection, Adjuvant, Saline, Control
